Building on learnings from Research Methods I, this course dives into laboratory DNA extraction protocols, how to quantify and ship DNA before reviewing DNA quality control methods and library preparation and sequencing of Oxford Nanopore before concluding with a look at GP2’s best practices for clinical data collection and storage using REDCap.
Modules:
- DNA Extraction and Quantification
- DNA Quality: purification, Femto pulse and tapestation
- Library Preparation and Sequencing for Oxford Nanopore
- Best practices for GP2 clinical data collection and storage using REDCap
Modules
In this first module, you will learn about laboratory DNA extraction protocols from different sample types, how to quantify DNA, how to ship DNA samples as well as some standard laboratory procedures when handling DNA.
This second module will walk you through an introduction to long read sequencing and high molecular DNA, followed by information on high molecular weight DNA extractions and quality control methods as well as tips for said quality control. Lastly, this module will briefly cover some DNA storage suggestions to help preserve DNA quality.
This module will walk you through long read sequencing preparation and sequencing for Oxford Nanopore. It will also cover basecalling options and a data analysis worflow generated by the CARD consortium that can be implemented on your data.
The last module of the course will introduce you to the REDCap platform and how you can use it for the collection, organisation and management of GP2 clinical data.
